General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress PTHR1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0118E2CP2L)
The cAMP Hunter PTHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. parathyroid hormone) based activation of PTHR1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR1, a naturally Gs coupled GPCR. Upon activation of PTHR1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM3 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0860E3CP0M)
The PathHunter CHRM3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM3 and EA-tagged β-Arrestin-2. Activation of CHRM3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems QuantiChrom™ Detergent Assay Kit. For quantitative determination of detergents in e.g. water and soil samples.
For quantitative determination of detergents in e.g. water and soil samples. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM. Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Method: 560nm and 650nm. Samples: Biological and environmental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.
Eurofins Discoverx PathHunter eXpress ACVRL1/BMPR2 Dimerization Assay, 2pl Kit. (93-0962E3CP22M)
The PathHunter ACVRL1/BMPR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/BMPR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVRL1 target protein fused to PK alongside BMPR2 target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/BMPR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR35 Activated GPCR Internalization Assay, 2pl Kit. (93-0968E3CP5M)
The PathHunter GPR35 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GPR35 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OXTR Activated GPCR Internalization Assay, 10pl Kit. (93-0715E3CP0L)
The PathHunter OXTR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OXTR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged OXTR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting OXTR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Rat IL-1B ASY
The R-PLEX Rat IL-1beta Assay from Meso Scale Discovery is a multiplex immunoassay that measures rat interleukin 1 beta (IL-1beta) in serum plasma tissue culture supernatants and other biological samples This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay can quantify rat IL-1beta concentrations between 0 06-1500 pg/mL with high precision and reproducibility This quantitative immunoassay allows simultaneous measurement of multiple analytes from a single sample enabling efficient and accurate cytokine profiling
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 2-Plate. (95-0118Y2-00057)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Abcam Xanthine/Hypoxanthine Assay Kit
Xanthine/Hypoxanthine Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress GPR39 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0338E2CP1L)
The PathHunter GPR39 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR39 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR39 and EA-tagged β-Arrestin-2. Activation of GPR39 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MRGPRF CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0433E2ACP2L)
The PathHunter MRGPRF eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of MRGPRF GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRF and EA-tagged β-Arrestin-2. Activation of MRGPRF stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Lonza Walkersville PyroGene Bulk Recombinant Factor C Endpoint Fluorescent Assay
The PyroGene Recombinant Factor C Assay is the evolution of endotoxin detection testing. Lonza scientists have developed a recombinant form of Factor C, the first component in the horseshoe crab clotting cascade activated by endotoxin.Recombinant Factor C(rFC) is activated by endotoxin binding, and the active enzyme then cleaves a synthetic substrate, resulting in the generation of a fluorogenic compound.PyroGene rFC works through a single enzymatic step compared to the multiple step enzymatic process necessary for LAL assays.Benefits:Sensitivity range from 0.005 to 5 EU/mlHigher endotoxin specificity through the elimination of false positive glucan reactionsLess lot-to-lot variabilitySecurity of supplyFDA acknowledged alternative to LALApplications:In-process testingFinal product release testingWater testingTesting plant-based materialContent:30 x 96 tests/vialrFC enzyme solution30 x 6 mL/vial fluorogenic substrate30 x 5 mL/vial rFC assay buffer10 vials endotoxin
Sigma Aldrich Fine Chemicals Biosciences In Vitro Toxicology Assay
Tox7 is a member of Sigmas CytoToxicity Kit series. It is based on the measurement of lactate dehydrogenase (LDH) release from cells as a measure of cell membrane damage and cytotoxicity. This technique has been utilized as an alternative to 51Cr release for cell mediated cytotoxicity assays as well as conventional cytotoxicity resulting from interaction of a test material with the cell.
Eurofins Discoverx PathHunter eXpress GPR61 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0407E2ACP1M)
The PathHunter GPR61 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR61 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR61 and EA-tagged β-Arrestin-2. Activation of GPR61 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL1R1/IL1RAP Dimerization Assay, 10pl Kit. (93-1032E3CP5L)
The PathHunter IL1R1/IL1RAP Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-1α) induced dimerization of IL1R1/IL1RAP receptors. This product uses EFC technology. The cell line included overexpresses IL1RAP target protein fused to PK alongside IL1R1 target protein fused to EA. Upon ligand-induced dimerization of IL1R1/IL1RAP, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.